The picture, which was taken by Prince William and features the Queen with her grandchildren and great-grandchildren, was taken during their time at Balmoral last summer.
However, many royal fans were quick to notice that Prince Harry and Meghan Markle's children, Archie and Lilibet, were not included in the photo.
The timing of the photo's release has raised some eyebrows as it came just a day before the coronation of King Charles.
Reports suggest that Prince William did not initially want to invite Prince Harry to the ceremony, so some have speculated that the release of the photo was an attempt to provoke Prince Harry before the event.
Overall, the absence of Prince Harry's children from the photo, combined with the timing of its release, has led some to believe that there may still be tension between the brothers.
